Research Biologist — Division of Biochemical Toxicology

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Lei Guo, Ph.D.
(870) 543-7121
[email protected]

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About  |  Publications  |  Lab Members

Background

Lei Guo received a Ph.D. degree in molecular biology from the University of Tokyo (Japan) in 1998, and worked as a postdoctoral fellow and then as a research assistant professor at the University of Alberta (Canada) for four years. Dr. Guo joined NCTR in 2003. She has published 96 articles in peer-reviewed journals and 16 book chapters. Her work has been cited more than 4000 times.

Research Interests

Dr. Guo's research focuses on mechanistic studies of drug (and herbal dietary supplement) associated toxicities. She uses multiple approaches, including, but not restricted to, molecular and biochemical methods, genomic analyses, and in silico molecular docking for the evaluation and prediction of toxicity and for the elucidation of toxic mechanisms. Her research results demonstrate that this combined approach is useful for hazard identification and that in-depth molecular mechanistic studies can provide toxicological information for FDA relevant-products. Dr. Guo has also developed in vitro methods to assess liver toxicity and for mechanistic investigations.

Professional Societies/National and International Groups

South Central Chapter, Society of Toxicology
Secretary
2011 – 2016

RC4 Undergraduate Award Selection Committee, Society of Toxicology
Member
2018

Board of Publication, Society of Toxicology
Member
2019 – Present

Selected Publications

The Role of Hepatic Cytochrome P450s in the Cytotoxicity of Sertraline. 
Chen S., Wu Q., Li X., Li D., Fan M., Ren Z., Bryant M., Mei N., Ning B., and Guo L. 
Arch Toxicol. 2020, doi: 10.1007/s00204-020-02753-y.
 

The Role of Hepatic Cytochrome P450s in the Cytotoxicity of Dronedarone.
Chen S, Wu Q, Ning B, Bryant MS, Guo L.
Arch Toxicol. 2018, 96 (6):1969-1981. doi:10.1007/s00204-018-2196-x
 

Mitochondrial Dysfunction Induced by Leflunomide and its Active Metabolite. 
Xuan J., Ren Z., Qing T., Couch L., Shi L., Tolleson WH., Guo L.
Toxicology. 2018, 396: 33-45. doi: 10.1016/j.tox.2018.02.003.
 

DNA Damage-Induced Apoptosis and Mitogen-Activated Protein Kinase Pathway Contribute to the Toxicity of Dronedarone in Hepatic Cells. 
Chen S., Ren Z., Yu D., Ning B., Guo L.
Environ Mol Mutagen. 2018, 59:278-289. doi: 10.1002/em.22173.
 

Endoplasmic Reticulum Stress and MAPK Signaling Pathway Activation Underlie Leflunomide-Induced Toxicity in HepG2 Cells.
Ren Z., Chen S., Qing T., Xuan J., Couch L., Yu D., Ning B., Shi L., Guo L.
Toxicology. 2017, 392:11-21. doi: 10.1016/j.tox.2017.10.002.
 

Activation of the Nrf2 Signaling Pathway in Usnic Acid-Induced Toxicity in HepG2 Cells.
Chen S., Zhang Z., Qing T., Ren Z., Yu D., Couch L., Ning B., Mei N., Shi L., Tolleson W., and Guo L.
Arch Toxicol. 2017, 91:1293-1307. doi: 10.1007/s00204-016-1775-y.
 

The Role of CYP 3A4 and 1A1 in Amiodarone-Induced Hepatocellular Toxicity.
Wu Q., Ning B., Xuan J., Ren Z., Guo L., and Bryant M.
Toxicol Lett. 2016, 253:55-62. doi: 10.1016/j.toxlet.2016.04.016.
 

Endoplasmic Reticulum Stress and Store-Operated Calcium Entry Contribute to Usnic Acid-Induced Toxicity in Hepatic Cells. 
Chen S., Zhang Z., Wu Y., Shi Q., Yan H., Mei N., Tolleson W., and Guo L.
Toxicol Sci. 2015, 146:116-126. doi: 10.1093/toxsci/kfv075.
 

Development of HepG2-Derived Cells Expressing Cytochrome P450s for Assessing Metabolism-Associated Drug-Induced Liver Toxicity. 
Xuan J., Chen S., Ning B., Tolleson W., and Guo L.
Chem Biol Interact. 2016, 5: 63-73. doi: 10.1016/j.cbi.2015.10.009.
 

The Role of Autophagy in Usnic Acid-Induced Toxicity in Hepatic Cells.
Chen S., Dobrovolsky V., Liu F., Wu Y., Zhang Z., Mei N., and Guo L.
Toxicol Sci. 2014, 142:33-44. doi: 10.1093/toxsci/kfu154.

Sertraline Induces Endoplasmic Reticulum Stress in Hepatic Cells.
Chen S., Xuan J., Couch L., Iyer A., Wu Y., Li Q., and Guo L.
Toxicology. 2014, 322C:78-88. doi: 10.1016/j.tox.2014.05.007.
 

Sertraline, an Antidepressant, Induces Apoptosis in Hepatic Cells through the Mitogen-Activated Protein Kinase Pathway. 
Chen S., Xuan J., Wan L., Lin H., Couch L., Mei N., Dobrovolsky V., and Guo L.
Toxicol Sci. 2014, 137:404-415. doi: 10.1093/toxsci/kft254.
 

Mechanism Study of Goldenseal-Associated DNA Damage..
Chen S., Wan L., Couch L., Lin H., Li Y., Dobrovolsky V., Mei N., and Guo L.
Toxicol Lett. 2013, 221:64-72. doi: 10.1016/j.toxlet.2013.05.641.
 

Mitochondrial Dysfunction Induced by Sertraline, an Antidepressant Agent. 
Li Y., Couch L., Higuchi M., Fang J., and Guo L.
Toxicol Sci. 2012, 127:582-591. doi: 10.1093/toxsci/kfs100.
 

Similarities and Differences in the Expression of Drug-Metabolizing Enzymes between Human Hepatic Cell Lines and Primary Human Hepatocytes.
Guo L., Dial S., Shi L., Branham W., Liu J., Fang J., Green B., Deng H., Kaput J., and Ning B.
Drug Metab Dispos. 2011, 39:528-538. doi: 10.1124/dmd.110.035873.
 

Methysticin and 7,8-Dihydromethysticin Are Two Major Kavalactonesin Kava Extract to Induce CYP1A1.
Li Y., Mei H., Wu Q., Zhang S., Fang J., Shi L., and Guo L.
Toxicol Sci. 2011, 124:388-399. doi: 10.1093/toxsci/kfr235.
 

Ginkgo Biloba Extract Induces Gene Expression Changes in Xenobiotics Metabolism and the Myc-Centered Network .
Guo L., Mei N., Liao W., Chan P., and Fu P.
OMICS. 2010, 14:75-90. doi: 10.1089/omi.2009.0115.
 

Gene Expression Profiling in Male B6C3F1 Mouse Livers Exposed to Kava Identifies--Changes in Drug Metabolizing Genes and Potential Mechanisms Linked to Kava Toxicity.
Guo L., Shi Q., Dial S., Xia Q., Mei N., Li Q., Chan P., and Fu P.
Food Chem Toxicol. 2010, 48:686-696. doi: 10.1016/j.fct.2009.11.050.
 

Analysis of Gene Expression Changes of Drug Metabolizing Enzymes in the Livers of F344 Rats Following Oral Treatment with Kava Extract. 
Guo L., Li Q., Xia Q., Dial S., Chan P., and Fu P.
Food Chem Toxicol. 2009, 47:433-442. PMID: 19100306.
 

Systematic and Simultaneous Gene Profiling of 84 Drug-Metabolizing Genes in Primary Human Hepatocytes. 
Ning B., Dial S., Sun Y., Wang J., Yang J., and Guo L.
J Biomol Screen. 2008, 13:194-201. doi: 10.1177/1087057108315513.
 

Rat Toxicogenomic Study Reveals Analytical Consistency Across Microarray Platforms. 
Guo L., Lobenhofer E., Wang C., Shippy R., Harris S., Zhang L., Mei N., Chen T., Herman D., Goodsaid F., Hurban P., Phillips K., Xu J., Deng X., Sun Y., Tong W., Dragan Y., and Shi L.
Nat Biotechnol. 2006, 24:1162-1169. doi: 10.1038/nbt1238.
 

Differences in Hepatotoxicity and Gene Expression Profiles by Anti-Diabetic PPAR Gamma Agonists on Rat Primary Hepatocytes and Human HepG2 Cells.
Guo L., Zhang L., Sun Y., Muskhelishvili L., Blann E., Dial S., Shi L., Schroth G., and Dragan Y.
Mol Divers. 2006, 10:349-360. PMID:17031537.
 

Lab Members

Contact information for all lab members:
(870) 543-7121
[email protected]

Si Chen, Ph.D.
Staff Fellow

Zhen Ren, Ph.D.
Staff Fellow