BAM Media M77: Lauryl Tryptose MUG (LST-MUG) Broth
January 2001
| Tryptose or trypticase | 20 g |
| Lactose | 5 g |
| K2HPO4 | 2.75 g |
| KH2PO4 | 2.75 g |
| NaCl | 5 g |
| Sodium lauryl sulfate | 0.1 g |
| 4-Methylumbelliferyl- β-D-glucuronide (MUG)* |
50 mg |
| Distilled water | 1 liter |
Prepare lauryl tryptose broth and add MUG. Dissolve with gentle heat if necessary. Dispense 10 ml portions into 20 × 150 mm test tubes containing inverted 10 × 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.
*MUG may be obtained from several sources, e.g., HACH Co., Loveland, CO.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.