BAM Media M145: Tetrathionate (TT) Broth
January 2001
Tetrathionate broth base
| Polypeptone | 5 g |
|---|---|
| Bile salts | 1 g |
| Calcium carbonate | 10 g |
| Sodium thiosulfate·5H2O | 30 g |
| Distilled water | 1 liter |
Suspend ingredients in 1 liter distilled water, mix, and heat to boiling. DO NOT AUTOCLAVE. (Precipitate will not dissolve completely.) Cool to less than 45°C. Store at 5-8°C. Final pH, 8.4 ± 0.2.
Iodine-Potassium Iodide (I2-KI) solution
| Potassium iodide | 5 g |
|---|---|
| Iodine, resublimed | 6 g |
| Distilled water, sterile | 20 ml |
Dissolve potassium iodide in 5 ml sterile distilled water. Add iodine and stir to dissolve. Dilute to 20 ml.
Brilliant green solution
| Brilliant green dye, sterile | 0.1 g |
|---|---|
| Distilled water, sterile | 100 m1 |
On day of use, add 20 ml I2-KI solution and 10 ml brilliant green solution to 1 liter base. Resuspend precipitate by gentle agitation and aseptically dispense 10 ml portions into 20 × 150 or 16 × 150 mm sterile test tubes. Do not heat medium after addition of I2-KI and dye solutions.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.