Staff Fellow — Division of Microbiology

Seongwon Nho, Ph.D.
(870) 543-7121
NCTRResearch@fda.hhs.gov

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About  |  Publications  |  Lab Members

Background

Dr. Seongwon Nho graduated with a Doctor of Veterinary Medicine degree from Gyeongsang National University, South Korea. Dr. Nho then earned a Ph.D. in veterinary medicine from Gyeongsang National University. In graduate school he studied proteomic response to infection in teleost fish and genome analysis of pathogens. He then trained at Mississippi State University as a postdoctoral fellow. He joined the Division of Microbiology at FDA’s National Center for Toxicological Research (NCTR) as an Oak Ridge Institute for Science and Education (ORISE) research fellow and has worked as a staff fellow in the same division since 2020. Dr. Nho received the FDA Leveraging Collaboration Award in 2019.

Research Interests   

Dr. Nho is experienced in biology techniques such as next generation sequencing, polymerase chain reaction usage, gene cloning and expression, electron microscopes, mass spectrometry, plaque assay, immunofluorescence assay, cell culture, and bioinformatics analysis. Dr. Nho’s current studies are focused on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Dr. Nho and his colleague generated recombinant SARS-CoV-2 spike glycoprotein reagents to study cell interactions and antibody-dependent enhancement. He and his colleague also are investigating the interaction between virus and host cells by generated pseudovirus expressing S, N, E, M, NSPs of SARS-CoV-2. He also seeks to understand calcium signaling that impacts the SARS-CoV-2 mediated cardiotoxicity.    

Professional Societies/National and International Groups

American Society for Microbiology
Member
2017– Present

Selected Publications

Pragmatic Strategy for Fecal Specimen Storage and the Corresponding Test Methods for Clostridioides difficile Diagnosis. 
Nho S.W., Kim M., Kim S.J., Foley S.L., Nayak R., Kweon O., and Cerniglia C.E. 
Pathogens. 2021, 10(8):1049. doi: 10.3390/pathogens10081049.

Microbial Contamination of Tattoo and Permanent Makeup Inks Marketed in the US: A Follow-Up Study. 
Nho S.W., Kim M., Kweon O., Kim S.J., Moon M.S., Periz G., Huang M.J., Dewan K., Sadrieh N.K., and Cerniglia C.E. 
Lett Appl Microbiol. 2020, 71(4):351-358. doi: 10.1111/lam.13353. 

CYPminer: An Automated Cytochrome P450 Identification, Classification, and Data Analysis Tool for Genome Data Sets Across Kingdoms. 
Kweon O., Kim S.J., Kim J.H., Nho S.W., Bae D., Chon J., Hart M., Baek D.H., Kim Y.C., Wang W., Kim S.K., Sutherland J.B., and Cerniglia C.E. 
BMC Bioinformatics. 2020, 21(1):160. doi: 10.1186/s12859-020-3473-2.

Taxonomic and Functional Metagenomic Profile of Sediment From a Commercial Catfish Pond in Mississippi. 
Nho S.W., Abdelhamed H., Paul D., Park S., Mauel M.J., Karsi A., and Lawrence M.L. 
Front Microbiol. 2018, 9:2855. doi: 10.3389/fmicb.2018.02855. 

Microbiological Survey of Commercial Tattoo and Permanent Makeup Inks Available in the United States. 
Nho S.W., Kim S.J., Kweon O., Howard P.C., Moon M.S., Sadrieh N.K., and Cerniglia C.E. 
J Appl Microbiol. 2018, 124(5):1294-1302. doi: 10.1111/jam.13713.

Improving Safety of a Live Attenuated Edwardsiella ictaluri Vaccine Against Enteric Septicemia of Catfish and Evaluation of Efficacy. 
Nho S.W., Abdelhamed H., Karsi A., and Lawrence M.L. 
Vet Microbiol. 2017, 210:83-90. doi: 10.1016/j.vetmic.2017.09.004. 

Evaluation of Three Recombinant Outer Membrane Proteins, OmpA1, Tdr, and TbpA, as Potential Vaccine Antigens Against Virulent Aeromonas hydrophila Infection in Channel Catfish (Ictalurus punctatus). 
Abdelhamed H., Ibrahim I., Nho S.W., Banes M.M., Wills R.W., Karsi A., and Lawrence M.L. 
Fish Shellfish Immunol. 2017, 66:480-486. doi: 10.1016/j.fsi.2017.05.043. 

Comparative Genomics and Transcriptional Analysis of Flavobacterium columnare Strain ATCC 49512. 
Tekedar H.C., Karsi A., Reddy J.S., Nho S.W., Kalindamar S., and Lawrence M.L. 
Front Microbiol. 2017, 8:588. doi: 10.3389/fmicb.2017.00588.

Characterization of a Specific Monoclonal Antibody Against Immunoglobulin Light Kappa/L1 Chain in Olive Flounder (Paralichthys olivaceus). 
Kim Y.K., Lee J.S., Jung J.W., Hikima J.I., Ohtani M., Jang H.B., Nho S.W., Cha I.S., Park S.B., Lee J.H., Aoki T., and Jung T.S. 
Fish Shellfish Immunol. 2017, 60:88-96. doi: 10.1016/j.fsi.2016.11.023.

Development of an Immunochromatography Assay Kit for Rapid Detection of Ranavirus. 
Kim Y.R., Park S.B., Fagutao F.F., Nho S.W., Jang H.B., Cha I.S., Thompson K.D., Adams A., Bayley A., and Jung T.S. 
J Virol Methods. 2015, 223:33-9. doi: 10.1016/j.jviromet.2015.07.009.

Identification of High-Risk Listeria monocytogenes Serotypes in Lineage I (Serotype 1/2a, 1/2c, 3a and 3c) Using Multiplex PCR. 
Nho S.W., Abdelhamed H., Reddy S., Karsi A., and Lawrence M.L. 
J Appl Microbiol. 2015, 119(3):845-52. doi: 10.1111/jam.12876.

Comparative Genomic Characterization of Three Streptococcus parauberis Strains in Fish Pathogen, as Assessed by Wide-Genome Analyses. 
Nho S.W., Hikima J., Park S.B., Jang H.B., Cha I.S., Yasuike M., Nakamura Y., Fujiwara A., Sano M., Kanai K., Kondo H., Hirono I., Takeyama H., Aoki T., and Jung T.S. 
PLoS One. 2013, 8(11):e80395. doi: 10.1371/journal.pone.0080395. 

Heat Shock Protein Profiles on the Protein and Gene Expression Levels in Olive Flounder Kidney Infected with Streptococcus parauberis. 
Cha I.S., Kwon J., Park S.B., Jang H.B., Nho S.W., Kim Y.K., Hikima J., Aoki T., and Jung T.S. 
Fish Shellfish Immunol. 2013, 34(6):1455-62. doi: 10.1016/j.fsi.2013.03.355.

RNA-Seq-Based Metatranscriptomic and Microscopic Investigation Reveals Novel Metalloproteases of Neobodo sp. as Potential Virulence Factors for Soft Tunic Syndrome in Halocynthia roretzi. 
Jang H.B., Kim Y.K., Del Castillo C.S., Nho S.W., Cha I.S., Park S.B., Ha M.A., Hikima J., Hong S.J., Aoki T., and Jung T.S. 
PLoS One. 2012, 7(12):e52379. doi: 10.1371/journal.pone.0052379. 

Complete Genome Sequence and Immunoproteomic Analyses of the Bacterial Fish Pathogen Streptococcus parauberis. 
Nho S.W., Hikima J., Cha I.S., Park S.B., Jang H.B., del Castillo C.S., Kondo H., Hirono I., Aoki T., and Jung T.S. 
J Bacteriol. 2011, 193(13):3356-66. doi: 10.1128/JB.00182-11. 

Comparative Sequence Analysis of a Multidrug-Resistant Plasmid from Aeromonas hydrophila. 
Del Castillo C.S., Hikima J., Jang H.B., Nho S.W., Jung T.S., Wongtavatchai J., Kondo H., Hirono I., Takeyama H., and Aoki T. 
Antimicrob Agents Chemother. 2013, 57(1):120-9. doi: 10.1128/AAC.01239-12. 

Enhanced Reliability of Avian Influenza Virus (AIV) and Newcastle Disease Virus (NDV) Identification Using Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry (MALDI-MS). 
Jang H.B., Sung H.W., Nho S.W., Park S.B., Cha I.S., Aoki T., and Jung T.S. 
Anal Chem. 2011, 83(5):1717-25. doi: 10.1021/ac102846q. 

Innate Immune Response in the Hemolymph of an Ascidian, Halocynthia roretzi, Showing Soft Tunic Syndrome, Using Label-Free Quantitative Proteomics. 
Cha I.S., Castillo C.S., Nho S.W., Hikima J., Aoki T., and Jung T.S. 
Dev Comp Immunol. 2011, 35(8):809-16. doi: 10.1016/j.dci.2011.01.011.

Lab Members 

Contact information for all lab members:
(870) 543-7391
NCTRResearch@fda.hhs.gov  

Marli Azevedo, Ph.D.
Research biologist

Lisa Mullis
Biologist