Staff Fellow — Genetic and Molecular Toxicology

Xiaoqing Guo, Ph.D.
(870) 543-7121
[email protected]  

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About  |  Publications  |  Lab Member

Background

Xiaoqing Guo received her master’s degree in pathology and a Ph.D. degree in medical sciences from Hebei Medical University in China in 1999 and 2005, respectively. She started her scientific career in 1999 in clinical cancer research, including diagnosis and treatment of early cancers as a physician in the 4th Affiliated Hospital of Hebei Medical University (also known as the Hebei Cancer Institute). Her Ph.D. training extended her career into molecular-biomarker screening for esophageal cancer in a high-risk area of China.

In June of 2008, Dr. Guo was recruited as a postdoctoral fellow in the Division of Genetic and Molecular Toxicology at NCTR. Following her postdoctoral training, Dr. Guo was hired as a research associate for Toxicologic Pathology Associates, Inc. (TPA). In January 2012, Dr. Guo joined NCTR as an FDA staff fellow. She has authored or co-authored more than 50 papers. Currently, she serves on the editorial and scientific review boards of several toxicology journals.

Research Interests

Since joining NCTR in 2008, Dr. Guo has worked intently to evaluate the mutagenicity and molecular-mechanistic analysis of chemicals of FDA regulatory interest using the in vitro genetic-toxicity assays included in the standard test battery. Dr. Guo is also an expert in conducting in vitro mouse lymphoma assay, the micronucleus assay, the Comet assay, in vivo mutation assays, and pathway-based mechanistic studies. She has used these assays to evaluate the mutagenicity of a variety of preparations of cigarette smoke (smoke condensates, whole-smoke solutions, whole smoke, and individual agents representing different chemical classes contained in cigarette smoke), nanomaterials, botanicals and mixtures, industrial compounds, and retail products.

Dr. Guo’s current research interest is developing pathway-based, medium- or high-throughput, and high-content (HTHC) approaches for genotoxicity testing. She also is interested in employing quantitative dose-response modeling approaches to evaluate the genotoxic dose-response relationships. Dr. Guo has successfully analyzed dose-response mouse-lymphoma assay data using several point-of-departure metrics, including benchmark dose and mutagenic potency. The conjunction of HTHC genetic-toxicity data and quantitative approaches provides a more efficient and streamlined process that may result in better regulatory decisions.

A major shortcoming of many previous studies is that assays were conducted in rodent- or human-cell lines without metabolic competence. Dr. Guo is interested in establishing appropriate in vitro cell models with metabolic competence, with the aim of generating data more relevant to human in vivo exposures. The resulting data may potentially be used to predict in vivo genotoxicity and for setting margin-of-exposure values to establish safe exposures levels for humans. Using the HTHC quantitative genotoxicity assays, Dr. Guo is collaborating with the Genetic Toxicology Group at the National Institute of Environmental Health Sciences/National Toxicology Program to test a number of chemicals in the Tox 21 library that have unusual responses observed in quantitative high-throughput screening assays.

Professional Societies/National and International Groups

Environmental Mutagenesis and Genomics Society (EMGS)
Member
2009 – Present          

Society of Toxicology (SOT)
Member
2009 – Present

South Central Regional Chapter of SOT
Member
2012 – Present
 
Councilor
2015 – 2016 

Select Publications

Publication titles are linked to text abstracts on PubMed.

Comparative Genotoxicity of TEMPO and Three of its Derivatives in Mouse Lymphoma Cells.
Guo X., Seo J.E., Bryce S.M., Tan J.A., Wu Q., Dial S.L., Martha M.M., and Mei N.
Toxicol Sci. 2018, 163(1): 214–225.
 

Quantitative Differentiation of Whole Smoke Solution-Induced Mutagenicity in the Mouse Lymphoma Assay.
Guo X., Heflich R.H., Dial S.L., De M., Richter P.A., and Mei N.
Environ Mol Mutagen. 2018, 59:103-113.
 

ROS Generation and JNK Activation Contribute to 4-Methoxy-TEMPO-Induced Cytotoxicity, Autophagy, and DNA Damage in HepG2 Cells.
Zhang Z., Ren Z., Chen S., Guo X., Guo L., and Mei N.
Arch Toxicol. 2018, (92):717-728.
 

Size- and Coating-Dependent Cytotoxicity and Genotoxicity of Silver Nanoparticles Evaluated Using In Vitro Standard Assays. 
Guo X., Li Y., Yan J., Ingle T.M., Jones M.Y., Mei N., Boudreau M.D., Cunningham K.C., Paredes A., Zhou T., Moore M.M., Howard P.C., and Chen T.
Nanotoxicology. 2016, 10(9):1373-1384.
 

Aloe vera: A Review of Toxicity and Adverse Clinical Effects.
Guo X. and Mei N.
J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2016, 34(2): 77-96.
 

Quantitative Analysis of In Vitro Mutagenicity Induced by Five Chemical Constituents of Tobacco Smoke.
Guo X., Dial S.L., Heflich R.H., Richter P.A., Moore M.M., and Mei N.
Mutagenesis. 2016, 31: 287–296.
 

Acetyl L-Carnitine Targets Adenosine Triphosphate Synthase in Protecting Zebrafish Embryos from Toxicities Induced by Verapamil and Ketamine: An In Vivo Assessment.
Guo X., Dumas M., Robinson B.L., Ali S.F., Paule M.G., Gu Q., and Kanungo J.
J Appl Toxicol. 2016, doi: 10.1002/jat.3340.
 

Developmental Toxicity Assay Using High Content Screening of Zebrafish Embryos.
Lantz-McPeak S., Guo X., Cuevas E., Dumas M., Newport G.D., Ali S.F., Paule M.G., and Kanungo J.
J Appl Toxicol. 2015, 35(3):261-72. 
 

Reactive Oxygen Species and C-Jun N-Terminal Kinases Contribute to TEMPO-Induced Apoptosis in L5178Y Cells.
Guo X., Chen S., Zhang Z., Dobrovolsky V.N., Dial S.L., Guo L., and Mei N.
Chem Biol Interact. 2015, 235: 27-36.
 

Ginkgo biloba Leaf Extract Induces DNA Damage by Inhibiting Topoisomerase II Activity in Human Hepatic Cells.
Zhang Z., Chen Si., Mei H., Xuan J., Guo X., Couch L., Guo L., and Mei N.
Sci Rep. 2015, 5: 14633.
 

Mutant Frequency in Comparison to Oxidative DNA Damage Induced by Ochratoxin A in L5178Y Tk+/- (3.7.2C) Mouse Lymphoma Cells.
Ali R., Guo X., Lin H., Ismail M., Khan Q.M., and Bhalli J.A.
Drug Chem Toxicol. 2014, 37(2): 227-232.
 

Assessment of the Toxic Potential of Grapheme Family Nanomaterials.
Guo X. and Mei N.
J Food Drug Anal. 2014, 22:105-115.
 

Mechanistic Evaluation of Ginkgo biloba Leaf Extract-Induced Genotoxicity in L5178Y Cells.
Lin H., Guo X., Zhang S., Dail S.L., Guo L., Manjanatha M.G., Moore M.M., and Mei N.
Toxicol Sci. 2014, 139(2): 338-349.
 

Nitroxide TEMPO: A Genotoxic and Oxidative Stress Inducer in Cultured Cells.
Guo X., Mittelstaedt R.A., Guo L., Shaddock J.G., Heflich R.H., Bigger A., Moore M.M., and Mei N.
Toxicol In Vitro. 2013, 27(5): 1496-1502.
 

Acetyl L-Carnitine Protects Motor Neurons and Rohon-Beard Sensory Neurons Against Ketamine-Induced Neurotoxicity in Zebrafish Embryos.
Cuevas E., Trickler W.J., Guo X., Ali S.F., Paule M.G., and Kanungo J.
Neurotoxicol Teratol. 2013, 39: 69-76.
 

Ketamine Attenuates Cytochrome P450 Aromatase Gene Expression and Estradiol-17 Levels in Zebrafish Early Life Stages.
Trickler W.J., Guo X., Cuevas E., Ali S.F., Paule M.G., and Kanungo J.
J Appl Toxicol. 2013, 34(5): 480-488.
 

Mutagenicity and DNA Adduct Formation by Aristolochic Acid in the Spleen of Big Blue Rats.
McDaniel P.L., Elander E., Guo X., Chen T., Arlt V.M., and Mei N.
Environ Mol Mutagen. 2012, 53(5): 358-368.
 

Nicotine Alters the Expression of Molecular Markers of Endocrine Disruption in Zebrafish.
Kanungo J., Cuevas E., Guo X., Lopez A.G., Ramirez-Lee M.A., Trickler W.,  Paule M.G., and Ali S.F.
Neuroscience Letters. 2012, 526 (2): 133-137.
 

Silver Nanoparticles Induced Genotoxicity and Oxidative Stress in Mouse Lymphoma Cells.
Mei N., Zhang Y., Chen Y., Guo X., Ding W., Ali S.F., Biris A.S., Rice A., Moore M.M., and Chen T.
Environ Mol Mutagen. 2012, 53: 409-419.
 

Mutagenicity of 11 Cigarette Smoke Condensates in Two Versions of the Mouse Lymphoma Assay.
Guo X., Verkler T.L., Chen Y., Richter P.A., Polzin G.M., Moore M.M., and Mei N.
Mutagenesis. 2011, 26(2): 273-281.
 

The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats.
Mei N., McDaniel L.P., Dobrovolsky V.N., Guo X., Shaddock J.G., Mittelstaedt R.A., Azuma M., Shelton S.D., McGarrity L.J., Doerge D.R., and Heflich RH.
Toxicol Sci. 2010, 115(2): 412-421.
 

Lab Member

Ji-Eun Soe, Ph.D.  
ORISE Fellow
(870) 543-7121
[email protected]