An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

In this section: Laboratory Methods (Food)

BAM Media M192c: Modified Buffered Peptone Water (mBPW)

Media Index for BAM

May 2024

Peptone	10.0 g
Sodium Chloride	5.0 g
Disodium Phosphate (Na2HPO4)	7.0 g
Monopotassium Phosphate (KH2PO4)	3.0 g
Distilled water	1000 ml

Autoclave at 121°C for 15 min. Final pH 7.2±0.2 at 25 °C

Note: Commercial Modified Buffered Peptone Water (Hardy Diagnostics CRITERIONTM; Catalog number C6351) or any other product which provides the same formulation as above may be substituted for the above formulation; Commercial Buffered Peptone Water (BPW; BD catalog number 218105) may be substituted for the above formulation by add 3.5 g of Na₂HPO₄ and 1.5 g of KH₂PO₄ to 20 g commercial BPW in 1000 ml distilled water.

Top