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In this section: Laboratory Methods (Food)

BAM Media M58: Ham's F-10 Medium

January 2001

Note: The commercial preparation is preferred.

L-Alanine	8.91 mg	Sodium pyruvate	110 mg
L-Arginine HCl	211.00 mg	Thymidine	0.727 mg
L-Asparagine·H₂O	15.00 mg	Biotin	0.024 mg
L-Aspartic acid	13.30 mg	Choline chloride	0.698 mg
L-Cysteine·2HCl	35.12 mg	Folic acid	1.320 mg
L-Glutamine	146.20 mg	Isoinositol	0.541 mg
L-Glutamic acid	14.70 mg	Niacinamide	0.615 mg
Glycine	7.51 mg	D-Calcium pantothenate	0.715 mg
L-Histidine HCl·H₂O	21.00 mg	Pyridoxine HCl	0.206 mg
L-Isoleucine	2.60 mg	Riboflavin	0.376 mg
L-Leucine	13.10 mg	Thiamine HCl	1.010 mg
L-Lysine·HCl	29.30 mg	Vitamin B₁₂	1.360 mg
L-Methionine	4.48 mg	CaCl₂·2H₂O	44.10 mg
D-Phenylalanine	4.96 mg	CuSO₄·5H₂O	0.0025 mg
L-Proline	11.50 mg	FeSO₄·7H₂O	0.83 mg
L-Serine	10.50 mg	KCl	285.00 mg
L-Threonine	3.57 mg	KH₂PO₄	83.00 mg
L-Tryptophan	0.60 mg	MgSO₄·7H₂O	152.80 mg
L-Tyrosine	1.81 mg	NaCl	7400.00 mg
L-Valine	3.50 mg	NaHCO₃	1200.00 mg
Glucose	1100.0 mg	NaH₂PO₄·H₂O	290.0 mg
Hypoxanthine	4.08 mg	ZnSO₄·7H₂O	0.028 mg
Lipoic acid	0.2 mg	Phenol red	1.2 mg
Distilled water			1 liter

Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.0 ± 0.2. Check sterility before use.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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